Re: Immunocytochemical Demonstration of Caseins in Mouse Mammary Glands
نویسندگان
چکیده
منابع مشابه
Selection and use of reference genes in mouse mammary glands.
Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most stable a...
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The major milk proteins, the caseins, contain multiple phosphorylation sites. Phosphorylation of the caseins is necessary to allow Ca2+ binding and aggregation of the caseins to form micelles. We have followed the phosphorylation of the caseins in isolated acini from lactating mouse mammary gland. Incubation of mammary cells with [32P]orthophosphate revealed that phosphorylation of newly synthe...
متن کاملImmunocytochemical demonstration of hormonally regulable casein in tumors produced by a rat mammary stem cell line.
The rat mammary epithelial stem cell line, Rat Mammary 25, and the dimethyl sulfoxide-resistant variant derived from it, Rama 259, when injected separately into female, virgin, athymic nude mice formed tumors which grew at approximately the same rate. When the mice were mated, the growth rate of the Rama 259 tumors increased 2.5-fold during late pregnancy and lactation, while that of the Rama 2...
متن کاملUltrasonographic Examination of Mammary Glands in Caspian Mares during the Lactation and Dry Period
Objective- The aim of this study was to determine the normal ultrasonographic and morphologic appearance of mammary gland and teat in Caspian mares during lactation and dry period. Design- Experimental study. Animals- Seven mature Caspian mares Procedures- Using a 6.5-8.5 MHz linear transducer the ultrasonographic appearance of lactating and dry mammary glands was determined in 7 mature Casp...
متن کاملImmunocytochemical demonstration of erythropoietin immunoreactivity in peritubular endothelial cells of the anemic mouse kidney.
Using a monoclonal antibody against human erythropoietin, its immunoreactivity was localized in the endothelial cells of peritubular blood vessels in the anemic mouse kidney. Other cell components of the kidney including glomeruli and tubules were negative. The non-anemic, normal kidney failed to reveal erythropoietin immunoreactivity.
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2002
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215540205000714